Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid
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چکیده
منابع مشابه
Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid
UNLABELLED Fast, definitive diagnosis of Creutzfeldt-Jakob disease (CJD) is important in assessing patient care options and transmission risks. Real-time quaking-induced conversion (RT-QuIC) assays of cerebrospinal fluid (CSF) and nasal-brushing specimens are valuable in distinguishing CJD from non-CJD conditions but have required 2.5 to 5 days. Here, an improved RT-QuIC assay is described whic...
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Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to t...
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Sporadic Creutzfeldt-Jakob disease (sCJD) is the commonest form of human prion diseases, accounting for about 85% of all cases. Current criteria for intra vitam diagnosis include a distinct phenotype, periodic sharp and slow-wave complexes at electroencephalography (EEG), and a positive 14-3-3-protein assay in the cerebrospinal fluid (CSF). In sCJD, the disease phenotype may vary, depending upo...
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Citation for published version: Stoeck, K, Sanchez-Juan, P, Gawinecka, J, Green, A, Ladogana, A, Pocchiari, M, Sanchez-Valle, R, Mitrova, E, Sklaviadis, T, Kulczycki, J, Slivarichova, D, Saiz, A, Calero, M, Knight, R, Aguzzi, A, Laplanche, J-L, Peoc'h, K, Schelzke, G, Karch, A, van Duijn, CM & Zerr, I 2012, 'Cerebrospinal fluid biomarker supported diagnosis of Creutzfeldt-Jakob disease and rapi...
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ژورنال
عنوان ژورنال: mBio
سال: 2015
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.02451-14